اخبار و اعلانات

 آمار

تعداد نشریات31
تعداد شماره‌ها850
تعداد مقالات8,200
تعداد مشاهده مقاله16,236,355
تعداد دریافت فایل اصل مقاله10,732,285
Sotudeh, Samira, Dehvari, Shahnaz, Mollaali, Milad, Bakhshipour, Alireza, Maleknejad, Abdulbaset, Kordi-Tamandani, Dor Mohammad, Zivarifar, Hamidreza. (1405). Predicting diagnostic values of expression levels of ERCC3-6, CHD1L, and ITGB7 genes in colorectal adenocarcinoma: A cross-sectional study with bioinformatic approaches. نشریه علمی دانشگاه سیستان و بلوچستان, (), -. doi: 10.22111/jep.2026.54572.1105
Samira Sotudeh; Shahnaz Dehvari; Milad Mollaali; Alireza Bakhshipour; Abdulbaset Maleknejad; Dor Mohammad Kordi-Tamandani; Hamidreza Zivarifar. "Predicting diagnostic values of expression levels of ERCC3-6, CHD1L, and ITGB7 genes in colorectal adenocarcinoma: A cross-sectional study with bioinformatic approaches". نشریه علمی دانشگاه سیستان و بلوچستان, , , 1405, -. doi: 10.22111/jep.2026.54572.1105
Sotudeh, Samira, Dehvari, Shahnaz, Mollaali, Milad, Bakhshipour, Alireza, Maleknejad, Abdulbaset, Kordi-Tamandani, Dor Mohammad, Zivarifar, Hamidreza. (1405). 'Predicting diagnostic values of expression levels of ERCC3-6, CHD1L, and ITGB7 genes in colorectal adenocarcinoma: A cross-sectional study with bioinformatic approaches', نشریه علمی دانشگاه سیستان و بلوچستان, (), pp. -. doi: 10.22111/jep.2026.54572.1105
Sotudeh, Samira, Dehvari, Shahnaz, Mollaali, Milad, Bakhshipour, Alireza, Maleknejad, Abdulbaset, Kordi-Tamandani, Dor Mohammad, Zivarifar, Hamidreza. Predicting diagnostic values of expression levels of ERCC3-6, CHD1L, and ITGB7 genes in colorectal adenocarcinoma: A cross-sectional study with bioinformatic approaches. نشریه علمی دانشگاه سیستان و بلوچستان, 1405; (): -. doi: 10.22111/jep.2026.54572.1105

Predicting diagnostic values of expression levels of ERCC3-6, CHD1L, and ITGB7 genes in colorectal adenocarcinoma: A cross-sectional study with bioinformatic approaches

Journal of Epigenetics
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 16 تیر 1405
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22111/jep.2026.54572.1105
نویسندگان
Samira Sotudeh1؛ Shahnaz Dehvari1؛ Milad Mollaali1؛ Alireza Bakhshipour2؛ Abdulbaset Maleknejad3؛ Dor Mohammad Kordi-Tamandani1؛ Hamidreza Zivarifar* 4
1Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
2Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran
3Department of General Surgery, Clinical Immunology Research Center, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran
4Department of Internal Medicine, Clinical Immunology Research Center, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran
چکیده
Dysregulation of DNA repair-related genes plays a crucial role in colorectal cancer development. The Excision Repair Cross-Complementation (ERCC) gene family, CHD1L, and ITGB7 are directly or indirectly related to DNA repair mechanisms. This study aimed to evaluate the putative diagnostic values of ERCC3, ERCC4, ERCC5, ERCC6, CHD1L, and ITGB7 genes in patients with colorectal adenocarcinoma (COAD). In this cross-sectional study, paired tumor and adjacent non-tumor colon tissues were collected from 20 adult patients with pathologically confirmed COAD between June 2022 and March 2023 in Zahedan, Iran. Differential expression was assessed using real-time quantitative PCR (RT-qPCR). Data were analyzed using SPSS 27 and GraphPad Prism 10.2.3. Complementary bioinformatics analyses were performed using GTEx, UALCAN, and the Human Protein Atlas databases. The experimental results showed that ERCC4 (+16.37%, p < 0.001), ERCC5 (+18.66%, p < 0.001), and ERCC6 (+8.78%, p < 0.001) were significantly upregulated in tumor tissues, while ERCC3 (-9.05%, p < 0.001), CHD1L (-11.48%, p < 0.001), and ITGB7 (-4.59%, p < 0.001) were downregulated. In tumor samples, only a significant positive correlation was observed between ERCC5 and ITGB7 (r = 0.59, p = 0.006). The receiver operating characteristic (ROC) curves predicted that all genes have significant diagnostic values for CRC; ERCC4 (area under the ROC curve (AUC) = 0.915) and ERCC5 (AUC = 0.958) were “outstanding”; CHD1L (AUC = 0.846) was “excellent”; ERCC3 (AUC = 0.713), ERCC6 (AUC = 0.784), and ITGB7 (AUC = 0.711) were “acceptable”. Overall, our experimental and bioinformatics findings suggested that the expression profiles of ERCC3, ERCC4, ERCC5, ERCC6, CHD1L, and ITGB7 exhibit strong diagnostic potential relevance in COAD. However, further functional studies are required to uncover these mechanisms.
کلیدواژه‌ها
Colorectal neoplasms؛ Gene expression؛ Quantitative PCR؛ DNA repair؛ Biomarkers؛ Iran
آمار
تعداد مشاهده مقاله: 146


سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب