اخبار و اعلانات

 آمار

تعداد نشریات28
تعداد شماره‌ها876
تعداد مقالات8,480
تعداد مشاهده مقاله17,289,902
تعداد دریافت فایل اصل مقاله11,604,933
Badakhshan, Yadollah, Yousefi, Manuchehr. (1405). Expression Profiles of Serotonin Receptor Genes in in Vitro-Produced Bovine Blastocysts: Insights from Microarray Analysis. نشریه علمی دانشگاه سیستان و بلوچستان, (), -. doi: 10.22111/jep.2026.54216.1102
Yadollah Badakhshan; Manuchehr Yousefi. "Expression Profiles of Serotonin Receptor Genes in in Vitro-Produced Bovine Blastocysts: Insights from Microarray Analysis". نشریه علمی دانشگاه سیستان و بلوچستان, , , 1405, -. doi: 10.22111/jep.2026.54216.1102
Badakhshan, Yadollah, Yousefi, Manuchehr. (1405). 'Expression Profiles of Serotonin Receptor Genes in in Vitro-Produced Bovine Blastocysts: Insights from Microarray Analysis', نشریه علمی دانشگاه سیستان و بلوچستان, (), pp. -. doi: 10.22111/jep.2026.54216.1102
Badakhshan, Yadollah, Yousefi, Manuchehr. Expression Profiles of Serotonin Receptor Genes in in Vitro-Produced Bovine Blastocysts: Insights from Microarray Analysis. نشریه علمی دانشگاه سیستان و بلوچستان, 1405; (): -. doi: 10.22111/jep.2026.54216.1102

Expression Profiles of Serotonin Receptor Genes in in Vitro-Produced Bovine Blastocysts: Insights from Microarray Analysis

Journal of Epigenetics
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 آبان 1405
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22111/jep.2026.54216.1102
نویسندگان
Yadollah Badakhshan* 1؛ Manuchehr Yousefi2
1Assistant professor Department of Animal Science, Faculty of Agriculture, University of Jiroft,
2Department of Animal Science, Faculty of Agriculture, University of Saravan, Saravan, Iran
چکیده
The increase of embryo production in in vitro fertilization is a primary objective of assisted reproductive technology. To this end, the use of various hormones in culture media and analyses of global gene expression serve as valuable tools for understanding the genetic mechanisms that may improve early embryo development. In this context, the serotonergic pathway is a widely studied factor in cell differentiation. Accordingly, this study aimed to investigate the gene expression of serotonergic receptors in blastocysts following thyroid hormone treatment, using microarray data from the GEO dataset GSE58901. Sixteen receptor subtypes were expressed in all blastocysts. Of these, eleven subtypes—HTR2A, HTR2B, HTR2C, HTR3A, HTR3C, HTR3E, HTR4, HTR5A, HTR6, HTR7, and SLC6A4 showed significantly elevated log-fold change (log-FC) in the treatment group compared to controls (P < 0.001). Among the expressed serotonin-related genes, HTR3A, SLC6A4, and HTR7 exhibited the highest log-FC values. Within the gene network, HTR3A and SLC6A4 displayed the most connections, each interacting with seven nodes, whereas HTR4 and HTR6 showed the fewest. Enrichment pathway analysis highlighted involvement in serotonin receptor signaling, G-protein-coupled receptor signaling, and related activity. In conclusion, serotonin receptor genes in bovine blastocysts showed a positive fold-change in expression, and the inclusion of thyroid hormones in the culture medium increased the log-FC of these genes. Notably, HTR3A and SLC6A4 demonstrated both the highest connectivity in the gene network and the greatest log-FC values. This suggests that HTR3A and SLC6A4 engage in extensive crosstalk with other receptors in the serotonergic system and interact with numerous intracellular signaling proteins in the bovine blastocyst
کلیدواژه‌ها
5-hydroxy-tryptophan؛ receptor؛ Micro-array؛ blastocyst؛ Cattle؛ IVF؛ thyroid hormones
آمار
تعداد مشاهده مقاله: 1


سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب