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Evaluation of Promoter Hypermethylation, Expression and, Oxytocin Receptor Gene (OXTR) Polymorphism with the Risk of Oral Squamous Cell Carcinoma | ||
| Journal of Epigenetics | ||
| دوره 2، شماره 1، فروردین 2021، صفحه 39-46 اصل مقاله (1.5 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22111/jep.2021.35782.1026 | ||
| نویسندگان | ||
| Behnaz Hassanpour1؛ Mohammad Ayoub Rigi-Ladiz* 2؛ Tayebeh Baranzehi1؛ Mohammad Javad Ashraf3؛ DorMohammad Kordi-Tamandani1 | ||
| 1Departement of Biology, University of Sistan and Baluchestan, Zahedan, Iran | ||
| 2Oral and Dental Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 3Department of Pathology, University of Medical Sciences, Shiraz | ||
| چکیده | ||
| Background: Oral cancer refers to a subgroup of head and neck’s neoplasm. It is estimated that about 90% of oral cancer is composed of OSCC. It frequently influences adult men, in particular, consumers of alcohol and tobacco users. OXTR is known as a G-protein receptor with seven transmembrane domains. G-proteins phosphatidylinositol-calcium as second messenger accomplishes its activity. PKC pathway activates through the receptor, in which the pathway has a role in cell proliferation and contraction. Material and methods: The present study was done to evaluate methylation, expression, and polymorphism of oxytocin receptor gene in both patients who suffer from OSCC and healthy samples. Promoter methylation status of the OXTR gene was evaluated in 163 samples using MSP-PCR. Also, OXTR mRNA expression profiles were also considered in 23 OSCC cases and 20 controls, by real-time PCR. The present study evaluated the association of the gene polymorphisms, rs2254298 and, rs53576, by using RFLP-PCR technique, with the risk of OSCC.Results: Promoter methylation assessment revealed a significant relationship between normal samples and patients. Our data showed that there is no significant linkage between occurrences of these single nucleotide polymorphisms and the risk of OSCC. Regarding allele frequency, no statistically significant differences were observed for A and G of rs2254298 and rs53576 genes between OSCC patients and healthy groups. The relative expression of OXTR mRNA in cases showed no significant differences compared to controls The main purpose of this research was to evaluate the correlation of OXTR gene polymorphisms rs53576 rs2254298 methylation, and expression pattern of this gene with the development of OSCC. Conclusion: OXTR methylation gene has an important role in performing OSCC. | ||
| کلیدواژهها | ||
| OSCC؛ Oxytocin receptor gene؛ Expression؛ Methylation Specific PCR؛ RFLP-PCR؛ rs2254298؛ rs53576 | ||
| مراجع | ||
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Bell, A. F., et al. (2015). Interaction between ox- ytocin receptor DNA methylation and genotype is associated with risk of postpartum depression in women without depression in pregnancy. Front Genet, 6: 243.
Ben Lerman, Trisheena Harricharran, & Olor- unseun O Ogunwobi. (2018). Oxytocin and cancer: An emerging link. World J Clin Oncol, 9(5): 74–82.
Blanks, A. M., & Thornton, S. (2003). The role of oxytocin in parturition. BJOG, 20: 46-51
Bockaert, J., & Pin, J. P. (1999). Molecular tinkering of G protein-coupled receptors: an evolutionary success. EMBO J, 18(8): 1723-1729.
Cassoni, P., et al. (2006). Oxytocin induces proliferation and migration in immortalized human dermal microvascular endothelial cells and human breast tumor-derived endothelial cells. Mol Cancer Res, 4(6), 351-359.
Cassoni, P., et al. (2001). Biological relevance of oxytocin and oxytocin receptors in cancer cells and primary tumors." Ann Oncol, 2:S37-S39.
Cassoni, P., et l. (2004). Oxytocin and oxytocin receptors in cancer cells and proliferation. J Neuroendocrinol, 16:362-364.
Cattaneo, M. G., Lucci, G., & Vicentini, L. M. (2009). Oxytocin stimulates in vitro angiog- enesis via a Pyk-2/Src-dependent mechanism. Exp Cell Res, 315(18), 3210-321.
Chatterjee, O., et al. (2016). An overview of the oxytocin-oxytocin receptor signaling network. J Cell Commun Signal, 10(4): 355-360.
Copland, J. A., et al. (1999). Demonstration of functional oxytocin receptors in human breast Hs578T cells and their up-regulation through a protein kinase C-dependent pathway. Endoc- rinology, 140(5): 2258-2267.
Das, P. M., and Singal, R. (2004). DNA methylation and cancer. J Clin Oncol, 22(22): 4632-4642.
Déry, M. C., et al. (2011). Oxytocin increases invasive properties of endometrial cancer cells through phosphate- idylinositol 3-kinase/AKT-dependent up-regula- tion of cyclooxygenase-1, -2, and X-linked inhibitor of apoptosis protein. Biol Reprod, 85(6): 1133-1142.
Gimpl, G., & Fahrenholz, F. (2001). The oxytocin receptor system: structure, function, and regulation. Physiol Rev, 81(2): 629-683.
Huan Xu., et al. (2017). The function of oxytocin: a potential biomarker for prostate cancer diagnosis and promoter of prostate cancer. Oncotarget, 8:31215-31226.
Jack, A., Connelly, J. J., & Morris, J. P. (2012). DNA methylation of the oxytocin receptor gene predicts neural response to ambiguous social stimuli. Front Hum Neurosci, 6:280.
Kimmel, M., et al. (2016). Oxytocin receptor DNA methy- lation in postpartum depression. Psychoneuroe-ndocr- inology, 69: 150-160.
Kim, S. H., et al. (2015). Oxytocin activates NF-κB-mediated inflammatory pathways in human gestational tissues. Mol Cell Endocrinol, 403:64-7.
Kimura, T., et al. (2003). Molecular regulation of the oxytocin receptor in peripheral organs. J Mol Endocrinol, 30(2):109-115.
Kraai jenvanger., et al. (2108) Epigenetic variability in the human oxytocin receptor (OXTR) gene: A possible pathway from early life experiences to psychopathologies. Neurosci- ence and Biobehavioral Reviews, 96: 127–142.
Kumsta, R., et al. (2013). Epigenetic regulation of the oxytocin receptor gene: implications for behavioral neuroscience. Front Neurosci, 7: 83.
Li J, et al. (2015). Association of Oxytocin Receptor Gene (OXTR) rs53576 Polymorphism with Sociality: A Meta-Analysis. PLoS One, 10(6).
Magon, N., & Karla, S. (2011). The orgasmic history of oxytocin: Love, lust, and labor. Indian J Endocrinol Metab, 13:156-161.
Markopoulos, A. K. (2012). Current Aspects on Oral Squamous Cell Carcinoma. The Open Dentistry Journal, 6:126-130.
Marocchio, L., et al. (2010). Oral squamous cell carcinoma: an analysis of 1,564 cases showing advances in early detection. Journal of Oral Science, 52: 267-273.
Massano, J., et al. (2006). Oral squamous cell carcinoma: review of prognostic and predictive factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102: 67-76.
Nishimori, K., et al. (2008). New aspects of oxytocin receptor function revealed by knockout mice: sociosexual behaviour and control of energy balance. Prog Brain Res, 170:79-90.
Rigi-Ladiz, M. A., et al. (2019). DNA methylation and expression status of glutamate receptor genes in patients with oral squamous cell carcinoma. Meta Gene, 20:100545.
Smearman, E. L., Yu, T., & Brody, G. H. (2016). Variation in the oxytocin receptor gene moderates the protective effects of a family-based prevention program on telomere length. Brain Behav, 6(2).
Takayanagi, Y., et al. (2005). Pervasive social deficits, but normal parturition, in oxytocin receptor-deficient mice. Proc Natl Acad Sci U S A, 102(44): 16096-16101.
Tsantoulis, P., et al.(2007). Advances in the biology of oral cancer. Oral Oncology, 43: 523-534.
Yang, J. Yu., et al. (2019). Intranasal Administr- ation of Oxytocin Attenuates Stress Responses Following Chronic Complicated Stress in Rats. Neurogastroenterol Motil, 25(4): 611-622.
Yu H, Liu G, Zhao P, & Zhu, L. (2011). Hormonal and reproductive factors and risk of esophageal cancer in Chinese postmenopausal women: a casecontrol study. Asian Pac J Cancer Prev , 12: 1953-1956.
Zhong, M., et al. (2010). Oxytocin induces the migration of prostate cancer cells: involvement of the Gi-coupled signaling pathway. Mol Cancer Res, 8(8): 1164-1172. | ||
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