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Vitamin D-Induced Cytotoxicity in HT-29 Colon Cancer Cells: Apoptotic Gene Expression Study | ||
| Journal of Epigenetics | ||
| دوره 7، شماره 1، تیر 2026، صفحه 21-27 اصل مقاله (563.49 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22111/jep.2026.53863.1100 | ||
| نویسندگان | ||
| Seyedeh Maedeh Hashemi؛ Hadi Habibollahi* ؛ Mehrnaz Ahmad Sharbafi | ||
| Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran. | ||
| چکیده | ||
| Background: Colorectal cancer (CRC) remains a major cause of cancer deaths worldwide, emphasizing the need for new treatments. Although vitamin D is known to impact cell growth and apoptosis, its precise mechanisms in affecting CRC and regulating apoptosis-related genes are not fully understood. This study investigated the possible cytotoxicity of vitamin D on human colon adenocarcinoma HT-29 cells and examined changes in BAX and BCL-2 gene expression. Methods: HT29 cells were cultured and exposed to vitamin D at concentrations ranging from 50 to 3750 µg/ml for 24 hours, with untreated cells as controls. Cell viability was assessed using an MTT assay, followed by RNA extraction. The RNA was reverse-transcribed into cDNA, allowing quantitative measurement of BAX and BCL-2 transcripts through real-time PCR, with data normalized to GAPDH. Statistical analysis of cell viability was performed using one-way ANOVA and Dunnett's post-hoc tests, while differences in gene expression were examined with t-tests. Results: Vitamin D decreased HT-29 cell viability in a dose-dependent and nonlinear way (p < 0.0001), with the highest inhibition seen at concentrations between 250-500 µg/ml. However, there was no significant difference in BAX expression (p = 0.1431) or BCL-2 expression (p = 0.5943). Conclusions: These data indicate that Vitamin D can cause cytotoxicity in HT-29 cells without altering the transcriptional targets BAX/BCL-2, and may also induce cell death through other pathways such as inhibited proliferation. Overall, this suggests that Vitamin D has potential association as a complementary therapy for CRC and warrants further mechanistic research, including studies with in vivo CRC models. | ||
| کلیدواژهها | ||
| Vitamin D؛ Colorectal cancer؛ Cytotoxicity؛ Apoptosis؛ BAX؛ BCL-2 | ||
| مراجع | ||
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