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Ebrahim, Shiva, Mehrabi, Fatemeh, Ebrahimi, Ahmad. (1399). Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF. نشریه علمی دانشگاه سیستان و بلوچستان, 1(2), 25-30. doi: 10.22111/jep.2020.30380.1014
Shiva Ebrahim; Fatemeh Mehrabi; Ahmad Ebrahimi. "Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF". نشریه علمی دانشگاه سیستان و بلوچستان, 1, 2, 1399, 25-30. doi: 10.22111/jep.2020.30380.1014
Ebrahim, Shiva, Mehrabi, Fatemeh, Ebrahimi, Ahmad. (1399). 'Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF', نشریه علمی دانشگاه سیستان و بلوچستان, 1(2), pp. 25-30. doi: 10.22111/jep.2020.30380.1014
Ebrahim, Shiva, Mehrabi, Fatemeh, Ebrahimi, Ahmad. Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF. نشریه علمی دانشگاه سیستان و بلوچستان, 1399; 1(2): 25-30. doi: 10.22111/jep.2020.30380.1014

Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF

Journal of Epigenetics
مقاله 4، دوره 1، شماره 2، تابستان 2019، صفحه 25-30  XML اصل مقاله (158.91 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22111/jep.2020.30380.1014
نویسندگان
Shiva Ebrahim1؛ Fatemeh Mehrabi2؛ Ahmad Ebrahimi email 3
1Department of Biology, central Tehran branch, Islamic Azad University, Tehran, Iran
2Infertility center of Tehran Yas hospital, medical school of Tehran University of medical science, Tehran, Iran
3Kowsar Human Genetics Research Institute, Yas medical Genetics Lab, Tehran, Iran
چکیده
1) Background: In vitro fertilization (IVF) is offered as a treatment for infertility in women.
. The Cell free DNA in plasma/follicles were suggested as a biomarker for embryo quality in
IVF. Higher the levels of Cell-free DNA indicate poorer embryo quality in the follicular fluid
during IVF. Therefore, this study was designed to examine the cell-free DNA (cfDNA) in
plasma and Fluid-follicle of women undergoing IVF-embryo transfer. 2) Methods: The study
examined 100 women undergoing IVF treatment in an IVF unit in Yas Hospital of Tehran
University of Medical Sciences in Tehran-Iran. Cell-free DNA was extracted from plasma
and follicular fluid of patients using the NucleoSpin® Extraction Kit. The total cfDNA was
examined by qPCR for ALBUMIN and GAPDH genes. 3) Results: we did not find a
statistically significant association among the variables such as CT-plasma, CT-follicular
fluid, delta-CT and average of these data between two groups (including patients who had a
successful IVF cycle and those who had a failed IVF cycle). 4) Conclusion: Plasma and
follicular fluid cfDNA may reflect the presence of factors which interfere with embryo
implantation. Further research is required to determine the usefulness of cfDNA as a
biomarker of IVF outcome and to examine the underlying pathologies as potential sources
for increased plasma/follicular fluid cfDNA concentrations.
کلیدواژه‌ها
Cell-Free DNA؛ Follicular fluid؛ plasma؛ IVF؛ ALBUMIN؛ GAPDH
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