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Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF | ||
| Journal of Epigenetics | ||
| مقاله 4، دوره 1، شماره 2، تیر 2019، صفحه 25-30 اصل مقاله (158.91 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22111/jep.2020.30380.1014 | ||
| نویسندگان | ||
Shiva Ebrahim1؛ Fatemeh Mehrabi2؛ Ahmad Ebrahimi  3
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| 1Department of Biology, central Tehran branch, Islamic Azad University, Tehran, Iran | ||
| 2Infertility center of Tehran Yas hospital, medical school of Tehran University of medical science, Tehran, Iran | ||
| 3Kowsar Human Genetics Research Institute, Yas medical Genetics Lab, Tehran, Iran | ||
| چکیده | ||
| 1) Background: In vitro fertilization (IVF) is offered as a treatment for infertility in women. . The Cell free DNA in plasma/follicles were suggested as a biomarker for embryo quality in IVF. Higher the levels of Cell-free DNA indicate poorer embryo quality in the follicular fluid during IVF. Therefore, this study was designed to examine the cell-free DNA (cfDNA) in plasma and Fluid-follicle of women undergoing IVF-embryo transfer. 2) Methods: The study examined 100 women undergoing IVF treatment in an IVF unit in Yas Hospital of Tehran University of Medical Sciences in Tehran-Iran. Cell-free DNA was extracted from plasma and follicular fluid of patients using the NucleoSpin® Extraction Kit. The total cfDNA was examined by qPCR for ALBUMIN and GAPDH genes. 3) Results: we did not find a statistically significant association among the variables such as CT-plasma, CT-follicular fluid, delta-CT and average of these data between two groups (including patients who had a successful IVF cycle and those who had a failed IVF cycle). 4) Conclusion: Plasma and follicular fluid cfDNA may reflect the presence of factors which interfere with embryo implantation. Further research is required to determine the usefulness of cfDNA as a biomarker of IVF outcome and to examine the underlying pathologies as potential sources for increased plasma/follicular fluid cfDNA concentrations. | ||
| کلیدواژه‌ها | ||
| Cell-Free DNA؛ Follicular fluid؛ plasma؛ IVF؛ ALBUMIN؛ GAPDH | ||
| مراجع | ||
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