
تعداد نشریات | 32 |
تعداد شمارهها | 739 |
تعداد مقالات | 7,170 |
تعداد مشاهده مقاله | 11,716,995 |
تعداد دریافت فایل اصل مقاله | 8,040,656 |
The relationship between FXIII Val34Leu and PAI-1 4G/5G gene polymorphisms and recurrent miscarriage in women from Golestan province | ||
Journal of Epigenetics | ||
دوره 3، شماره 1، خرداد 2022، صفحه 7-12 اصل مقاله (455.11 K) | ||
نوع مقاله: Original Article | ||
شناسه دیجیتال (DOI): 10.22111/jep.2022.40936.1036 | ||
نویسندگان | ||
Nazanin Ghaderi Nejad1؛ Fojan saboori2؛ Nader Mansour Samaei3؛ Dor Mohammad Kordi-Tamandani* 1 | ||
1Department of Biology, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, Iran | ||
2Department of Biology, Faculty of Basic Science, Zanjan Islamic Azad University, Zanjan, Iran | ||
3Department of Medical Genetics, Faculty of Advanced Medical Technologies and Golestan Research Center of Gastroenterology and Hepatotology, Golestan University of Medical Sciences, Gorgan, Iran | ||
چکیده | ||
The main purpose of this study was to work out the relationship between different polymorphisms of PAI (Plasminogen Activator Inhibitor type 1) 4G/5G and XIII Val34Leu genes in women with first-semester recurrent miscarriage (RM) syndrome. Recurrent miscarriage (RM) is an obstetric challenge. Polymorphisms of factor XIII (FXIII)and plasminogen activator inhibitor-1 (PAI-1) may cause an imbalance between coagulation and fibrinolysis that can end in RM. This case-control study enclosed 48 women with at least two or three abortions and 50 women with at least one pregnancy as a control. DNA molecule was extracted from peripheral blood samples by the phenol-chloroform methodology. Different variants of the two genes were amplified by Amplification Refractory Mutation System - Polymerase chain reaction (ARMS-PCR) method. Finally, we analyzed allele frequencies and genotypes, Odd Ratios, Chi-square, Fisher's and Students T-test for the data. During this study, it has been found that 50% of the case population have the normal genotype for the PAI-1 (rs1799762) gene, 31.25% had a heterozygous genotype and 18.75% had a mutant homozygote. The frequency of the mutated allele in the patient population compared to the controls have p-values <0.001 and it is statistically significant for this allele (OR = 0.068, p-values <0.001, CI = 0.014-0.322). In contrast, no significant results were observed for the factor XIII (rs5985) gene and this variant was considered in this population as an ineffective polymorphism on recurrent miscarriage syndrome (p-values = 0.238). Finally, it is suggested that other variants of the given gene should be examined in Golestan. | ||
کلیدواژهها | ||
PAI-1 4G/5G؛ FXIIIa Val34Leu؛ Thrombophilia؛ Recurrent miscarriage؛ ARMS PCR | ||
مراجع | ||
Adler, G., E. Mahmutbegovic, A. Valjevac, M. A. Adler, N. Mahmutbegovic, K. Safranow, E. Czerska, A. Pawinska-Matecka, I. Ciechanowicz And D. Marjanovic (2018). "Association Between-675 Id, 4g/5g Pai-1 Gene Polymorphism And Pregnancy Loss: A Systematic Review." Acta Informatica Medica 26(3): 156.
Al Sallout, R. J. And F. A. Sharif (2010). "Polymorphisms In Nos3, Ace And Pai-1 Genes And Risk Of Spontaneous Recurrent Miscarriage In The Gaza Strip." Medical Principles And Practice 19(2): 99-104.
Aragon, T. J., M. P. Fay, D. Wollschlaeger, A. Omidpanah And M. A. Omidpanah (2017). "Package ‘Epitools’."
Bagheri, M., I. A. Rad, M. D. Omrani And F. Nanbaksh (2011). "The Val34leu Genetic Variation In The A Subunit Of Coagulation Factor Xiii In Recurrent Spontaneous Abortion." Systems Biology In Reproductive Medicine 57(5): 261-264.
Bargahi, N., M. Farajzadeh, A. Poursadegh-Zonouzi And D. Farajzadeh (2014). "Prevalence Of Thrombophilic Gene Polymorphisms In An Azari Population Of Iran." Hematology Reports 6(2).
Burrows, R. F., J. Ray And E. A. Burrows (2000). "Bleeding Risk And Reproductive Capacity Among Patients With Factor Xiii Deficiency: A Case Presentation And Review Of The Literature." Obstetrical & Gynecological Survey 55(2): 103.
Cavalli-Sforza, L. L., L. Cavalli-Sforza, P. Menozzi And A. Piazza (1994). The History And Geography Of Human Genes, Princeton University Press.
Coulam, C. B., R. S. Jeyendran, L. A. Fishel And R. Roussev (2006). "Multiple Thrombophilic Gene Mutations Rather Than Specific Gene Mutations Are Risk Factors For Recurrent Miscarriage." American Journal Of Reproductive Immunology 55(5): 360-368.
Dardik, R., J. Loscalzo And A. Inbal (2006). "Factor Xiii (Fxiii) And Angiogenesis." Journal Of Thrombosis And Haemostasis 4(1): 19-25.
Dawson, S., A. Hamsten, B. Wiman, A. Henney And S. Humphries (1991). "Genetic Variation At The Plasminogen Activator Inhibitor-1 Locus Is Associated With Altered Levels Of Plasma Plasminogen Activator Inhibitor-1 Activity." Arteriosclerosis And Thrombosis: A Journal Of Vascular Biology 11(1): 183-190.
Elmahgoub, I. R., R. A. Afify, A. A. A. Aal And W. S. El-Sherbiny (2014). "Prevalence Of Coagulation Factor Xiii And Plasminogen Activator Inhibitor-1 Gene Polymorphisms Among Egyptian Women Suffering From Unexplained Primary Recurrent Miscarriage." Journal Of Reproductive Immunology 103: 18-22.
Muszbek, L., R. Adany And H. Mikkola (1996). "Novel Aspects Of Blood Coagulation Factor Xiii. I. Structure, Distribution, Activation, And Function." Critical Reviews In Clinical Laboratory Sciences 33(5): 357-421.
Parveen, F., M. Tuteja And S. Agrawal (2013). "Polymorphisms In Mthfr, Mthfd, And Pai-1 And Recurrent Miscarriage Among North Indian Women." Archives Of Gynecology And Obstetrics 288(5): 1171-1177.
Rai, R. And L. Regan (2006). "Recurrent Miscarriage." The Lancet 368(9535): 601-611.
Szecsi, P. B., M. Jørgensen, A. Klajnbard, M. R. Andersen, N. P. Colov And S. Stender (2010). "Haemostatic Reference Intervals In Pregnancy." Thrombosis And Haemostasis 103(04): 718-727.
Toth, B., U. Jeschke, N. Rogenhofer, C. Scholz, W. Würfel, C. J. Thaler And A. Makrigiannakis (2010). "Recurrent Miscarriage: Current Concepts In Diagnosis And Treatment." Journal Of Reproductive Immunology 85(1): 25-32.
Wickham, H. (2011). "Ggplot2." Wiley Interdisciplinary Reviews: Computational Statistics 3(2): 180-185. | ||
آمار تعداد مشاهده مقاله: 868 تعداد دریافت فایل اصل مقاله: 808 |