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Ashrafi, Hafizullah, Mohammadi, Mohammadullah, Hemati, Simin, Eisazaei, sepideh, Mollaali, Milad, Siahpoosh, Zakieh. (1401). Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province. نشریه علمی دانشگاه سیستان و بلوچستان, 3(2), 1-8. doi: 10.22111/jep.2022.41418.1039
Hafizullah Ashrafi; Mohammadullah Mohammadi; Simin Hemati; sepideh Eisazaei; Milad Mollaali; Zakieh Siahpoosh. "Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province". نشریه علمی دانشگاه سیستان و بلوچستان, 3, 2, 1401, 1-8. doi: 10.22111/jep.2022.41418.1039
Ashrafi, Hafizullah, Mohammadi, Mohammadullah, Hemati, Simin, Eisazaei, sepideh, Mollaali, Milad, Siahpoosh, Zakieh. (1401). 'Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province', نشریه علمی دانشگاه سیستان و بلوچستان, 3(2), pp. 1-8. doi: 10.22111/jep.2022.41418.1039
Ashrafi, Hafizullah, Mohammadi, Mohammadullah, Hemati, Simin, Eisazaei, sepideh, Mollaali, Milad, Siahpoosh, Zakieh. Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province. نشریه علمی دانشگاه سیستان و بلوچستان, 1401; 3(2): 1-8. doi: 10.22111/jep.2022.41418.1039

Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province

Journal of Epigenetics
دوره 3، شماره 2، آذر 2022، صفحه 1-8  XML اصل مقاله (333.73 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22111/jep.2022.41418.1039
نویسندگان
Hafizullah Ashrafi1؛ Mohammadullah Mohammadi1؛ Simin Hemati email orcid 2؛ sepideh Eisazaei1؛ Milad Mollaaliorcid 1؛ Zakieh Siahpooshorcid 1
1Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
2Department of Radiation Oncology, Isfahan University of Medical Science, Isfahan, Iran
چکیده
Objective: Gastric cancer (GC) is the fourth most common cancer worldwide, occurring in approximately two-thirds of developing countries, and is one of the leading causes of cancer deaths. The mitogen-activated protein kinase 14 (MAPK14) plays an important role in the conversion of extracellular stimuli to a wide range of cellular responses. The 5-hydroxytryptamine receptor 2B (HTR2B) increases the level of 5-HT; and as a result, platelet-derived 5-HT promotes tumor angiogenesis, tumor growth, and metastatic potential of cancer cells. Materials and Methods: Expression analysis was performed on 20 healthy cases and 20 cancerous cases for MAPK14 and 20 healthy cases and 19 cancerous cases for HTR2B using real-time quantitative PCR (qRT-PCR) assay. Polymorphisms were evaluated in 38 healthy cases and 30 cancerous cases for rs550352538, and 35 healthy cases and 35 cancerous cases for rs28763973 via tetra-primer amplification refractory mutation system PCR (T-ARMS PCR) technique. Results: No significant difference was observed in MAPK14 and HTR2B gene expression levels (P>0.05). In addition, there is no statistically significant association of different genotypes in rs28763973 from MAPK14 gene and rs550352538 from HTR2B gene with gastric cancer (P>0.05). Conclusions: No significant difference was noted between patient and healthy individuals in expression levels and gene polymorphisms; nevertheless, results may vary by significantly changing the gene pool or population size.
کلیدواژه‌ها
Stomach Neoplasms؛ Real-Time Polymerase Chain Reaction؛ Genetic Variation؛ Mitogen-Activated Protein Kinase 14؛ Receptor, Serotonin, 5-HT2B
مراجع

Browne, A., Göbel, A., Thiele, S., Hofbauer, L., Rauner, M., & Rachner, T. (2016). p38 MAPK regulates the Wnt inhibitor Dickkopf-1 in osteotropic prostate cancer cells. Cell death & disease, 7, e2119-e2119. doi:10.1038/cddis.2016.32.

Consortium, U. (2019). UniProt: a worldwide hub of protein knowledge. Nucleic acids research, 47, D506-D515. doi:10.1093/nar/gky1049.

Digklia, A., & Wagner, A. D. (2016). Advanced gastric cancer: Current treatment landscape and future perspectives. World journal of gastroenterology, 22, 2403-2414. doi:10.3748/wjg.v22.i8.2403.

Ebrahimi, M. E., Kordi-Tamandani, D. M., & Arish, M. (2016). A novel approach to investigation of the pathogenesis of pterygium based on assessment of promoter hyper-methylation and expression profile of CTLA4 gene: A credible report of CTLA4 gene expression in human eye tissue. Gene, 583, 130-133. doi:10.1016/j.gene.2016.02.027.

Eusebi, L. H., Telese, A., Marasco, G., Bazzoli, F., & Zagari, R. M. J. J. o. g. (2020). Gastric cancer prevention strategies: a global perspective. Journal of gastroenterology and hepatology, 35, 1495-1502. doi:10.1111/jgh.15037.

García-Hernández, L., García-Ortega, M. B., Ruiz-Alcalá, G., Carrillo, E., Marchal, J. A., & García, M. Á. (2022). The p38 MAPK Components and Modulators as Biomarkers and Molecular Targets in Cancer. International journal of molecular sciences, 23, 370. doi:10.3390/ijms23010370.

Johnston, F. M., & Beckman, M. (2019). Updates on management of gastric cancer. Current oncology reports, 21, 1-9. doi:10.1007/s11912-019-0820-4.

Karmakar, S., & Lal, G. (2021). Role of serotonin receptor signaling in cancer cells and anti-tumor immunity. Theranostics, 11, 5296–5312. doi:10.7150/thno.55986.

Katoh, H., & Ishikawa, S. (2021). Lifestyles, genetics, and future perspectives on gastric cancer in east Asian populations. Journal of human genetics, 66, 887-899. doi:10.1038/s10038-021-00960-8.

Li, Y., Kong, C., Wu, C., Wang, Y., Xu, B., Liang, S., & Ying, X. (2019). Knocking down of LINC01220 inhibits proliferation and induces apoptosis of endometrial carcinoma through silencing MAPK11. Bioscience reports, 39, BSR20181794. doi:10.1042/BSR20181794.

Padmanabhan, N., Ushijima, T., & Tan, P. (2017). How to stomach an epigenetic insult: the gastric cancer epigenome. Nature Reviews Gastroenterology & Hepatology, 14, 467-478. doi:10.1038/nrgastro.2017.53.

Peters, M. A., Meijer, C., Fehrmann, R. S., Walenkamp, A. M., Kema, I. P., de Vries, E. G., . . . Research, O. (2020). Serotonin and dopamine receptor expression in solid tumours including rare cancers. Pathology oncology research, 26, 1539-1547. doi:10.1007/s12253-019-00734-w.

Pruitt, K. D., Tatusova, T., & Maglott, D. R. J. N. a. r. (2005). NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Nucleic acids research, 33, D501-D504. doi:10.1093/nar/gki025.

Rawla, P., & Barsouk, A. (2019). Epidemiology of gastric cancer: global trends, risk factors and prevention. Przeglad gastroenterologiczny, 14, 26. doi:10.5114/pg.2018.80001.

Safran, M., Rosen, N., Twik, M., BarShir, R., Stein, T. I., Dahary, D., . . . Lancet, D. (2021). The GeneCards Suite. In I. Abugessaisa & T. Kasukawa (Eds.), Practical Guide to Life Science Databases (pp. 27-56). Singapore: Springer. doi:10.1007/978-981-16-5812-9_2.

Sexton, R. E., Al Hallak, M. N., Diab, M., & Azmi, A. S. (2020). Gastric cancer: a comprehensive review of current and future treatment strategies. Cancer metastasis reviews, 1-25. doi:10.1007/s10555-020-09925-3.

Sherry, S. T., Ward, M., & Sirotkin, K. (1999). dbSNP-database for single nucleotide polymorphisms and other classes of minor genetic variation. Genome research, 9, 677-679.

Sitarz, R., Skierucha, M., Mielko, J., Offerhaus, G. J. A., Maciejewski, R., & Polkowski, W. P. (2018). Gastric cancer: epidemiology, prevention, classification, and treatment. Cancer management and research, 10, 239-248. doi:10.2147/CMAR.S149619.

Spandidos, A., Wang, X., Wang, H., & Seed, B. (2010). PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic acids research, 38, D792-D799. doi:10.1093/nar/gkp1005.

Sudo, T., Yagasaki, Y., Hama, H., Watanabe, N., & Osada, H. (2002). Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells. Biochem Biophys Res Commun, 291, 838-843. doi:10.1006/bbrc.2002.6529.

Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin, 71, 209-249. doi:10.3322/caac.21660.

Tan, Z. (2019). Recent Advances in the Surgical Treatment of Advanced Gastric Cancer: A Review. Med Sci Monit, 25, 3537-3541. doi:10.12659/msm.916475.

Thrift, A. P., & El-Serag, H. B. (2020). Burden of gastric cancer. Clinical Gastroenterology and Hepatology, 18, 534-542. doi:10.1016/j.cgh.2019.07.045.

Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B. C., Remm, M., & Rozen, S. G. (2012). Primer3--new capabilities and interfaces. Nucleic Acids Res, 40, e115. doi:10.1093/nar/gks596.

Wang, F.-H., Zhang, X.-T., Li, Y.-F., Tang, L., Qu, X.-J., Ying, J.-E., . . . Xu, R.-H. (2021). The Chinese Society of Clinical Oncology (CSCO): Clinical guidelines for the diagnosis and treatment of gastric cancer, 2021. Cancer Commun (Lond), 41, 747-795. doi:10.1002/cac2.12193.

Yeoh, K. G., & Tan, P. (2021). Mapping the genomic diaspora of gastric cancer. Nature Reviews Cancer. doi:10.1038/s41568-021-00412-7.

Zhao, Y., Zhang, J., Cheng, A. S. L., Yu, J., To, K. F., & Kang, W. (2020). Gastric cancer: genome damaged by bugs. Oncogene, 39, 3427-3442. doi:10.1038/s41388-020-1241-4.

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