دانشگاه سیستان و بلوچستان

  اخبار و اعلانات

 آمار

تعداد نشریات33
تعداد شماره‌ها769
تعداد مقالات7,457
تعداد مشاهده مقاله12,441,418
تعداد دریافت فایل اصل مقاله8,469,517
Askari, Nahid, Parvizpour, Sepideh, Aghaabbasi, Kian, Hadizadeh, Morteza. (1402). Epigenetics, Pharmacoinformatics, and Experimentally Validation of Wnt- signaling in treatment of prostate cancer using Blepharis Persica. نشریه علمی دانشگاه سیستان و بلوچستان, 4(1), 32-24. doi: 10.22111/jep.2023.44839.1051
Nahid Askari; Sepideh Parvizpour; Kian Aghaabbasi; Morteza Hadizadeh. "Epigenetics, Pharmacoinformatics, and Experimentally Validation of Wnt- signaling in treatment of prostate cancer using Blepharis Persica". نشریه علمی دانشگاه سیستان و بلوچستان, 4, 1, 1402, 32-24. doi: 10.22111/jep.2023.44839.1051
Askari, Nahid, Parvizpour, Sepideh, Aghaabbasi, Kian, Hadizadeh, Morteza. (1402). 'Epigenetics, Pharmacoinformatics, and Experimentally Validation of Wnt- signaling in treatment of prostate cancer using Blepharis Persica', نشریه علمی دانشگاه سیستان و بلوچستان, 4(1), pp. 32-24. doi: 10.22111/jep.2023.44839.1051
Askari, Nahid, Parvizpour, Sepideh, Aghaabbasi, Kian, Hadizadeh, Morteza. Epigenetics, Pharmacoinformatics, and Experimentally Validation of Wnt- signaling in treatment of prostate cancer using Blepharis Persica. نشریه علمی دانشگاه سیستان و بلوچستان, 1402; 4(1): 32-24. doi: 10.22111/jep.2023.44839.1051

Epigenetics, Pharmacoinformatics, and Experimentally Validation of Wnt- signaling in treatment of prostate cancer using Blepharis Persica

Journal of Epigenetics
دوره 4، شماره 1، آذر 2023، صفحه 32-24    اصل مقاله (809.19 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22111/jep.2023.44839.1051
نویسندگان
Nahid Askari* 1؛ Sepideh Parvizpour2؛ Kian Aghaabbasi3؛ Morteza Hadizadeh4
1Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
2Research center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Biotechnology, University Campus 2 - University of Guilan, Rasht, Iran
4Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
چکیده
Wnt/β-catenin signaling is a pathway involved in epigenetics and cancer. It regulates cell processes and malignancy in prostate cancer cells. Wnt/β-catenin signaling is also influenced by epigenetic mechanisms, such as DNA methylation, histone modifications, and non-coding RNAs. Therefore, targeting Wnt/β-catenin signaling and epigenetics may be a promising strategy for prostate cancer therapy. Blepharis persica (B. persica) is a plant with phenolic and flavonoid compounds that has anti-cancer properties. However, its mechanisms of action are unclear.
We studied the effects of B. persica extract on Wnt/β-catenin signaling in prostate cancer cells (PC-3). We used MTT assay, GC-MS, RT-qPCR, and molecular docking to evaluate the cytotoxicity, composition, gene expression, and protein-ligand interactions of B. persica extract on PC-3 cells. We found that B. persica extract reduced PC-3 cell viability, down-regulated Wnt target genes (CTNNB1 and SNAIL), and up-regulated Wnt antagonists (APC and AXIN). Moreover, molecular docking analysis suggested that four compounds from B. persica extract, namely blepharone A, blepharone B, blepharone C, and blepharone D, had favorable binding energies and interactions with FZD7 protein. These results suggest that these compounds may act as potential inhibitors or modulators of FZD7 protein and thus affect the epigenetic regulation of Wnt signaling in PC-3 cells. These findings provide new insights into the molecular mechanism of B. persica extract on prostate cancer cells and suggest its potential as a natural modulator of Wnt signaling and epigenetics in prostate cancer therapy. epigenetics in PC-3 cells and may have therapeutic potential.
کلیدواژه‌ها
"PC-3 cells"؛ "Prostate cancer"؛ "Belpharis persica"؛ "FZD7"؛ "signaling pathway"
مراجع

Alizadeh, Anita, et al. "Crosstalk between long non-coding RNA DLX6-AS1, microRNAs and signaling pathways: A pivotal molecular mechanism in human cancers." Gene 769 (2021): 145224.

Blankesteijn, W.M., Interventions in WNT signaling to induce cardiomyocyte proliferation: crosstalk with other pathways. Molecular Pharmacology, 2020. 97(2): p. 90-101.

Castagnoli, L., et al., WNT signaling modulates PD-L1 expression in the stem cell compartment of triple-negative breast cancer. Oncogene, 2019. 38(21): p. 4047-4060.

Daulat, A.M. and J.-P. Borg, Wnt/planar cell polarity signaling: new opportunities for cancer treatment. Trends in cancer, 2017. 3(2): p. 113-125.

Dirar, A.I., et al., Genus Blepharis (Acanthaceae): a review of ethnomedicinally used species, and their phytochemistry and pharmacological activities. Journal of ethnopharmacology, 2021. 265: p. 113255.

Flores-Hernández, Eric, et al. "Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells." Cellular Signalling 72 (2020): 109636.

Gaikar, N., et al., Blepharis persica increases testosterone biosynthesis by modulating StAR and 3β-HSD expression in rat testicular tissues. Asian Pacific Journal of Reproduction, 2022. 11(1): p. 27.

Gaikar, N., et al., Isolation, characterization and estimation of benzoxazinoid glycoside from seeds of Blepharis persica (Burm. f) O. Kuntze. Separation Science Plus, 2021. 4(4): p. 163-173.

Gaikar, Nilesh, et al. "Blepharis persica increases testosterone biosynthesis by modulating StAR and 3β-HSD expression in rat testicular tissues. Asian Pacific Journal of Reproduction. 2022. 11(1) : p. 27.

Ghorbani, M., The economic geology of Iran. Mineral deposits and natural resources. Springer, 2013: p. 1-450.

Gupta, P.S.S., et al., Computer-aided discovery of bis-indole derivatives as multi-target drugs against cancer and bacterial infections: DFT, docking, virtual screening, and molecular dynamics studies. Journal of Molecular Liquids, 2020. 320: p. 114375.

Huggins, Ian J., et al. "The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells." Nature communications 8.1 (2017): 1034.

Hur, J. and E. Giovannucci, Racial differences in prostate cancer: does timing of puberty play a role? British Journal of Cancer, 2020. 123(3): p. 349-354.

Katoh, Masaru. "Canonical and non-canonical WNT signaling in cancer stem cells and their niches: Cellular heterogeneity, omics reprogramming, targeted therapy and tumor plasticity." International journal of oncology 51.5 (2017): 1357-1369.

Khan, I., et al. "Ethnobotany and medicinal uses of folklore medicinal plants belonging to family Acanthaceae: An updated review. MOJ Biol Med. 2017. 1(2): p. 34-38.

Kiełbowski, Kajetan, et al. "The role of selected non-coding RNAs in the biology of non-small cell lung cancer." Advances in Medical Sciences 68.1 (2023): 121-137.

Kuropakornpong, P., et al., In vitro and in vivo anti-inflammatory activities of Benjakul: a potential medicinal product from Thai traditional medicine. Evidence-Based Complementary and Alternative Medicine, 2020. 2020.

Liu, L.-J., et al., Aberrant regulation of Wnt signaling in hepatocellular carcinoma. World journal of gastroenterology, 2016. 22(33): p. 7486.

Mottet, N., et al., EAU-EANM-ESTRO-ESUR-SIOG guidelines on prostate cancer—2020 update. Part 1: screening, diagnosis, and local treatment with curative intent. European urology, 2021. 79(2): p. 243-262.

Odero-Marah, V., et al., Epithelial-mesenchymal transition (EMT) and prostate cancer. Cell & Molecular Biology of Prostate Cancer, 2018: p. 101-110.

Paige Brock, A., et al., A novel gammaherpesvirus in a large flying fox (Pteropus vampyrus) with blepharitis. Journal of Veterinary Diagnostic Investigation, 2013. 25(3): p. 433-437.

Qiang, Y.-W., et al., Wnts induce migration and invasion of myeloma plasma cells. Blood, 2005. 106(5): p. 1786-1793.

Rawla, P., T. Sunkara, and V. Gaduputi, Epidemiology of pancreatic cancer: global trends, etiology and risk factors. World journal of oncology, 2019. 10(1): p. 10.

Reguart, N., et al., The role of Wnt signaling in cancer and stem cells. 2005.

Sharma, Uttam, et al. "Role of Hedgehog and Hippo signaling pathways in cancer: a special focus on non-coding RNAs." Pharmacological Research (2022): 106523.

Waddell, Nicholas J., et al. "Transcription and DNA methylation signatures of paternal behavior in hippocampal dentate gyrus of prairie voles." Scientific Reports 13.1 (2023): 11020.

Wang, Liang, et al. "GIPC2 interacts with Fzd7 to promote prostate cancer metastasis by activating WNT signaling." Oncogene 41.18 (2022): 2609-2623.

Wang, Zhuo, et al. "The Wnt signaling pathway in tumorigenesis, pharmacological targets, and drug development for cancer therapy." Biomarker Research 9 (2021): 1-16.

Zhan, T., et al., MEK inhibitors activate Wnt signalling and induce stem cell plasticity in colorectal cancer. Nature communications, 2019. 10(1): p. 1-17.

Zimmermann, M., Investigation to determine the role of the TCF/TLE family of transcription factors in stem cell self-renewal and differentiation in myeloid malignancies. 2015, Hochschule für angewandte Wissenschaften Hamburg.

آمار
تعداد مشاهده مقاله: 421
تعداد دریافت فایل اصل مقاله: 377


سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب