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Abolhasanzadeh Parizi, Ali, Lagzian, Milad. (1403). Immunomodulatory Effects of Ferula assa-foetida Extract on NF-κB and STAT1 Signaling Pathways in GM-CSF-Differentiated Human Macrophages. نشریه علمی دانشگاه سیستان و بلوچستان, 5(2), 41-52. doi: 10.22111/jep.2024.50612.1077
Ali Abolhasanzadeh Parizi; Milad Lagzian. "Immunomodulatory Effects of Ferula assa-foetida Extract on NF-κB and STAT1 Signaling Pathways in GM-CSF-Differentiated Human Macrophages". نشریه علمی دانشگاه سیستان و بلوچستان, 5, 2, 1403, 41-52. doi: 10.22111/jep.2024.50612.1077
Abolhasanzadeh Parizi, Ali, Lagzian, Milad. (1403). 'Immunomodulatory Effects of Ferula assa-foetida Extract on NF-κB and STAT1 Signaling Pathways in GM-CSF-Differentiated Human Macrophages', نشریه علمی دانشگاه سیستان و بلوچستان, 5(2), pp. 41-52. doi: 10.22111/jep.2024.50612.1077
Abolhasanzadeh Parizi, Ali, Lagzian, Milad. Immunomodulatory Effects of Ferula assa-foetida Extract on NF-κB and STAT1 Signaling Pathways in GM-CSF-Differentiated Human Macrophages. نشریه علمی دانشگاه سیستان و بلوچستان, 1403; 5(2): 41-52. doi: 10.22111/jep.2024.50612.1077

Immunomodulatory Effects of Ferula assa-foetida Extract on NF-κB and STAT1 Signaling Pathways in GM-CSF-Differentiated Human Macrophages

Journal of Epigenetics
دوره 5، شماره 2، بهمن 2024، صفحه 41-52    اصل مقاله (725.46 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22111/jep.2024.50612.1077
نویسندگان
Ali Abolhasanzadeh Parizi1؛ Milad Lagzian* 2
1Dept. of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
2دانشکده علوم، گروه زیست شناسی، دانشگاه سیستان و بلوچستان
چکیده
The immunomodulatory properties of Ferula assa-foetida, a perennial herb traditionally used for its anti-inflammatory effects, were investigated in this study, focusing on its influence on NF-κB and STAT1 signaling pathways in GM-CSF-differentiated human macrophages. The extract was prepared using 80% ethanol, and its cytotoxicity was evaluated using the MTT assay, revealing no significant cytotoxic effects at concentrations ranging from 10 to 200 µg/mL. Differentiated macrophages were treated with the extract, and quantitative real-time PCR (qRT-PCR) was employed to assess the expression levels of NF-κB and STAT1 genes. The results demonstrated a dose-dependent downregulation of NF-κB expression by the extract, with significant reductions observed at concentrations of 50 µg/mL and higher. Specifically, NF-κB expression decreased to 0.6719 ± 0.0691-fold at 50 µg/mL (p = 0.0044), 0.3959 ± 0.1170-fold at 100 µg/mL (p = 0.0009), and 0.1650 ± 0.0509-fold at 200 µg/mL (p < 0.0001). In contrast, STAT1 expression remained unaffected across all tested concentrations of the extract, with no statistically significant changes compared to the untreated control group. Lipopolysaccharide (LPS), used as a positive control, significantly upregulated both NF-κB and STAT1 expression, confirming the responsiveness of the macrophages to inflammatory stimuli. These findings suggest that the extract selectively modulates NF-κB-dependent inflammatory pathways without broadly affecting STAT1 signaling. The study highlights the potential of F. assa-foetida as a source of bioactive compounds with anti-inflammatory properties. However, further research is needed to elucidate the underlying molecular mechanisms, identify the active compounds, and validate these effects in vivo.
کلیدواژه‌ها
Ferula assa-foetida؛ NF-κB؛ STAT1؛ GM-CSF؛ macrophages؛ qRT-PCR؛ immunomodulation
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