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Comparative effects of Mesembryanthemum nodiflorum extract and biosynthesized silver nanoparticles on the expression of biofilm-related genes in Klebsiella pneumoniae isolated from urinary tract infections in Zahedan (2022-2023) | ||
| Journal of Epigenetics | ||
| دوره 5، شماره 2، بهمن 2024، صفحه 53-60 اصل مقاله (320.73 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22111/jep.2025.50974.1079 | ||
| نویسنده | ||
| Ali Qasemi* | ||
| Assistant professor, Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran. | ||
| چکیده | ||
| Urinary tract infections (UTIs) are widespread bacterial infections, exacerbated by high recurrence rates and antibiotic resistance, particularly due to multidrug-resistant (MDR) Klebsiella pneumoniae (K. pneumoniae). This pathogen’s ability to form biofilms significantly enhances its persistence and resistance to treatment. This study, explored the antimicrobial and antibiofilm effects of Mesembryanthemum nodiflorum (M. nodiflorum) extract and its biosynthesized silver nanoparticles (AgNPs) on MDR, biofilm-forming K. pneumoniae strains from UTI patients in Zahedan, Iran. Of 91 bacterial isolates, 68 were confirmed as K. pneumoniae strains, with 87% producing biofilms and 63% being strong biofilm formers. HPLC analysis of the extract identified bioactive compounds like phenolic acids and flavonoids. AgNPs synthesized using the extract were spherical (25–50 nm) and characterized through UV-Vis spectroscopy, TEM, SEM, and XRD. The antimicrobial efficacy of M. nodiflorum extract and AgNPs was assessed using the disk diffusion method and minimum inhibitory concentration (MIC) determination, while antibiofilm activity was evaluated using the microtiter plate assay. The impact on biofilm-related gene expression was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). AgNPs exhibited superior antimicrobial activity with a significantly lower MIC (32 µg/mL) compared to the extract (256 µg/mL). At sub-MIC concentrations, the extract (128 µg/mL) and AgNPs (16 µg/mL) reduced biofilm biomass by 51% and 73%, respectively. Gene expression analysis revealed that AgNPs significantly downregulated biofilm-related genes (rmpA: 34.44%, fimH: 54.2%, and mrkA: 46.42%) compared to the extract (rmpA: 11.77%, fimH: 16.84%, and mrkA: 15.52%). These findings highlight the potential of M. nodiflorum bioactive compounds and AgNPs as alternative therapeutic agents for managing biofilm-associated UTIs caused by MDR K. pneumoniae. The study provides valuable insights into the mechanisms underlying biofilm inhibition and supports the development of novel, eco-friendly, and effective treatment strategies to combat resistant uropathogens. | ||
| کلیدواژهها | ||
| Urinary tract infections؛ Klebsiella pneumoniae؛ Antibiofilm activity؛ Silver nanoparticles؛ Mesembryanthemum nodiflorum | ||
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آمار تعداد مشاهده مقاله: 274 تعداد دریافت فایل اصل مقاله: 128 |
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